HspA1L Overexpression Lysate (Native)

Images

 
Western Blot: HspA1L Overexpression Lysate (Adult Normal) [NBL1-11750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HspA1L.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HspA1L Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205351 and based on accession number NM_005527. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens heat shock 70kDa protein 1-like (HSPA1L), mRNA.
Gene
HSPA1L

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
70.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HspA1L

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HspA1L Overexpression Lysate (Native)

  • heat shock 10kDa protein 1-like
  • Heat shock 70 kDa protein 1-Hom
  • Heat shock 70 kDa protein 1L
  • heat shock 70 kDa protein 1-like
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
  • HSP70-1L
  • HSP70-HOM
  • HSP70T
  • hum70t

Background

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HspA1L Lysate (NBL1-11750) (0)

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Bioinformatics

Gene Symbol HSPA1L