Human HSP90 alpha ELISA Kit (Colorimetric)

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ELISA: Human HSP90 alpha ELISA Kit (Colorimetric) [NBP2-29914]
ELISA: Human HSP90 alpha ELISA Kit (Colorimetric) [NBP2-29914] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell lysate, serum and plasma (citrate, heparin)
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.03 ng/mL

Order Details

Human HSP90 alpha ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human HSP90 alpha. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human HSP90 alpha positive sample. No cross-reactivity or interference detected.
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.03 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8.6%
Spike Recovery
Please See Protocol for Specific Spike Recovery Details
Sample Volume
100 uL (prediluted 1:25)
Kit Type
ELISA Kit (Colorimetric)
Gene
HSP90AA1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
The human Hsp90A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Hsp90A.
Publications
Read Publication using
NBP2-29914 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human HSP90 alpha
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human HSP90 alpha monoclonal antibody
  5. Human HSP90 alpha Standard lyophilized, 20 ng/mL upon reconstitution
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Streptavidin-HRP
  9. Substrate Solution (tetramethyl-benzidine)
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human HSP90 alpha ELISA Kit (Colorimetric)

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • HSP86
  • Hsp89
  • HSP89A
  • HSP90 alpha
  • Hsp90
  • HSP90A
  • HSP90AA1
  • HSP90AHSP86
  • HSP90N
  • HSPC1
  • HSPC1HSP 86
  • HSPCA
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Background

The human HSP90 alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human HSP90 alpha.

HSP90 alpha is a molecular chaperone that belongs to the heat shock protein 90 family predominantly known in helping in the refolding of misfolded proteins or their elimination in case of irreversible damage. The name is simply derived from the molecular weight roughly being 90 Kilo Daltons. There are two existing isoforms, Heat Shock Protein 90 alpha (HSP90a) and Heat Shock Protein 90 beta (HSP90B), with 76% homology. HSP90 is a very abundant continuously expressed protein also involved in non-stress related processes such as structural maintenance, cell cycle control and cell signalling. The protein contains three functional domains, the ATP-binding, protein binding and dimerizing domain all essential for the cytoprotecitve properties of HSP90.

HSP90 has emerged as a potential target for cancer therapy as many tumor cells such as non-small cell lung cancer, oesophageal squamous cell carcinoma, pancreatic carcinoma and malignant melanoma overexpress HSP90alpha. The activity of many oncogenic proteins depend on HSP90 and anti HSP90alpha drugs have become an exciting area in the search for cancer therapies and other diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for HSP90 alpha ELISA Kit (NBP2-29914)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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Hsp90A - helping to fold a wide variety of signal transduction proteins
Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the...  Read full blog post.

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Bioinformatics

Gene Symbol HSP90AA1