Human HSP90 alpha ELISA Kit (Colorimetric)

ELISA: Human HSP90 alpha ELISA Kit (Colorimetric) [NBP2-29914]

ELISA: Human HSP90 alpha ELISA Kit (Colorimetric) [NBP2-29914] - Standard curve

Product Discontinued

Human HSP90 alpha ELISA Kit (Colorimetric) Summary

• Specificity: The assay detects both natural and recombinant human HSP90 alpha. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human HSP90 alpha positive sample. No cross-reactivity or interference detected.
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.03 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 8.6%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 100 uL (prediluted 1:25)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HSP90AA1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human Hsp90A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Hsp90A.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HSP90 alpha ELISA Kit (Colorimetric)

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• HSP86
• Hsp89
• HSP89A
• HSP90 alpha
• Hsp90
• HSP90A
• HSP90AA1
• HSP90AHSP86
• HSP90N
• HSPC1
• HSPC1HSP 86
• HSPCA
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

The human HSP90 alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human HSP90 alpha.

HSP90 alpha is a molecular chaperone that belongs to the heat shock protein 90 family predominantly known in helping in the refolding of misfolded proteins or their elimination in case of irreversible damage. The name is simply derived from the molecular weight roughly being 90 Kilo Daltons. There are two existing isoforms, Heat Shock Protein 90 alpha (HSP90a) and Heat Shock Protein 90 beta (HSP90B), with 76% homology. HSP90 is a very abundant continuously expressed protein also involved in non-stress related processes such as structural maintenance, cell cycle control and cell signalling. The protein contains three functional domains, the ATP-binding, protein binding and dimerizing domain all essential for the cytoprotecitve properties of HSP90.

HSP90 has emerged as a potential target for cancer therapy as many tumor cells such as non-small cell lung cancer, oesophageal squamous cell carcinoma, pancreatic carcinoma and malignant melanoma overexpress HSP90alpha. The activity of many oncogenic proteins depend on HSP90 and anti HSP90alpha drugs have become an exciting area in the search for cancer therapies and other diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HSP90 alpha ELISA Kit (NBP2-29914)(2)

Publications using NBP2-29914

• Ozbay Kurt FG, Lasser S, Arkhypov I et al. Enhancing immunotherapy response in melanoma: myeloid-derived suppressor cells as a therapeutic target The Journal of clinical investigation 2023-07-03 [PMID: 37395271]
• Balanescu A, Stan I, Codreanu I, et al. Circulating Hsp90 Isoform Levels in Overweight and Obese Children and the Relation to Nonalcoholic Fatty Liver Disease: Results from a Cross-Sectional Study Dis. Markers 2019-12-03 [PMID: 31885746] (ELISA, Human)

FAQs for HSP90 alpha ELISA Kit (NBP2-29914). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: HSP90AA1