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HSP90 alpha Antibody [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
NBP1-77685AF488
Alexa Fluor 647
NBP1-77685AF647
Alexa Fluor 700
NBP1-77685AF700
Biotin
NBP1-77685B
DyLight 405
NBP1-77685V
DyLight 488
NBP1-77685G
DyLight 550
NBP1-77685R
DyLight 650
NBP1-77685C
FITC
NBP1-77685F
HRP
NBP1-77685H
Unconjugated
NBP1-77685
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HSP90 alpha Antibody [Alexa Fluor® 405] Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Hsp90A protein (between residues 650-732) [UniProt P07900]
Localization
Cytoplasm. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSP90AA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This HSP90-1A antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections and Western blot where a band is seen ~84 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human, mouse, rat, and primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HSP90 alpha Antibody [Alexa Fluor® 405]

  • FLJ31884
  • Heat shock 86 kDa
  • heat shock 90kD protein 1, alpha
  • heat shock 90kD protein 1, alpha-like 4
  • heat shock 90kD protein, alpha-like 4
  • heat shock 90kDa protein 1, alpha
  • heat shock protein 90kDa alpha (cytosolic), class A member 1
  • heat shock protein HSP 90-alpha
  • HSP86
  • Hsp89
  • HSP89A
  • HSP90 alpha
  • Hsp90
  • HSP90A
  • HSP90AA1
  • HSP90AHSP86
  • HSP90N
  • HSPC1
  • HSPC1HSP 86
  • HSPCA
  • HSPCAHSP89A
  • HSPCAL1
  • HSPCAL4
  • HSPN
  • LAP2
  • Renal carcinoma antigen NY-REN-38

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: HspA4 Antibody [NBP1-81696] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: HspA4 Antibody [NBP1-81696] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Hsp90B2P Antibody
NBP1-02459
Immunohistochemistry-Paraffin: Hsp90B2P Antibody [NBP1-02459] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Immunohistochemistry-Paraffin: Hsp90B2P Antibody [NBP1-02459] - Human kidney
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
GR/NR3C1 Antibody
(BuGR2)
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(2 Reviews)
(7 Publications)
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
HSP60 Antibody
NBP1-77397
Western Blot: HSP60 Antibody [NBP1-77397] - Western blot analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000
Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
Immunohistochemistry: HSP60 Antibody [NBP1-77397] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
(4 Reviews)
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
HSP27 Antibody
NBP1-75477
Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Hsp27 Antibody [NBP1-75477] - analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
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(2 Publications)
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HSPA8/HSC71/Hsc70
Antibody (1B5)
NBP1-97868
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis using the Biotin conjugate of NBP1-97868. Detection of HSC70/HSP73, mAb (1B5) (biotin conjugate): Lane 1: HSC70/HSP73 (bovine), (rec); Lane 2: HSP70/HSP72 (human), (recombinant); Lane 3: 3T3 (heat shocked): Lane 4: PC-12 (heat shocked); Lane 5: CHO-K1 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked); Lane 8: Vero (heat shocked).
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.
Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).
(1 Review)
(10 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
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Immunohistochemistry: eNOS Antibody [NB300-500] - The pulmonary tissues were analyzed for expression of eNOS protein by immunohistochemistry(IHC). Dark brown staining of indicates eNOS-positive cells. IHC stain of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group (magnification, x400).
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
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Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
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p23/PTGES3
Antibody (JJ3)
NB300-576
Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.
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STI1 Antibody (4B6)
H00010963-M01
Western Blot: STI1 Antibody (4B6) [H00010963-M01] - STIP1 monoclonal antibody (M01), clone 4B6 Analysis of STIP1 expression in HeLa.
Immunocytochemistry/Immunofluorescence: STI1 Antibody (4B6) [H00010963-M01] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STI1 Antibody (4B6) [H00010963-M01] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.3 ug/ml.
(4 Publications)
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
ErbB2/Her2
[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129)
(5 Reviews)
(26 Publications)
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AGA Antibody
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Immunocytochemistry/Immunofluorescence: AGA Antibody [NBP1-88866] - Staining of human cell line U-2 OS shows positivity in vesicles and cytoskeleton (microtubules).
Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
GLS2 Antibody
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Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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FKBP52/FKBP4
Antibody
NBP1-84675
Western Blot: FKBP52/FKBP4 Antibody [NBP1-84675] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody [NBP1-84675] - Staining in human testis and duodenum tissues using anti-FKBP4 antibody. Corresponding FKBP4 RNA-seq data are presented for the same tissues.
(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P
Raf-1 Antibody
(563002)
MAB4540
Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf‑1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Raf‑1 was detected in immersion fixed paraffin-embedded sections of human liver array using Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu, Mu, Rt
Applications: WB, IHC
TPT1/TCTP Antibody
NBP2-41206
Western Blot: TPT1/TCTP Antibody [NBP2-41206] - Western blot analysis of TPT1 in human brain tissue lysate with TPT1 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry: TPT1/TCTP Antibody [NBP2-41206] - Immunohistochemistry of TPT1 in rat brain tissue with TPT1 antibody at 2.5 ug/ml.
Immunofluorescence: TPT1/TCTP Antibody [NBP2-41206] - TPT1 in rat brain tissue with TPT1 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
CDK5 Activator 1
Antibody (739304)
MAB7357
Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CDK5 Activator 1 Monoclonal Antibody (Catalog # MAB7357) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDK5 Activator 1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
Species: Hu
Applications: WB
RRAS Antibody
NB100-56161
RRAS Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Tmp21/p23 Antibody
NB110-57586
Western Blot: TMP21 Antibody [NB110-57586] - Chicken embryo extract - Data supplied by Dr Yonghua Zhang of McGill University.
Immunohistochemistry: TMP21 Antibody [NB110-57586] - Detection of TMP21 in central neurons.
Western Blot: TMP21 Antibody [NB110-57586] - Detection of TMP21 in human liver lysate.
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, IHC, IHC-P
gp96/HSP90B1/GRP94
Antibody (9G10)
NB300-619
Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-77685AF488
Alexa Fluor 647 Labeled NBP1-77685AF647
Alexa Fluor 700 Labeled NBP1-77685AF700
Learn about Custom Antibody Labeling

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Blogs on HSP90 alpha.

Check out the latest blog posts on HSP90 alpha.
Hsp90A - helping to fold a wide variety of signal transduction proteins
Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the...  Read full blog post.

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Bioinformatics

Gene Symbol HSP90AA1
Entrez