HSP90 alpha Antibody

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NBP1-02459] - Staining of human testis tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Product Discontinued

HSP90 alpha Antibody Summary

• Immunogen: Synthetic peptide from human HSP90 alpha corresponding to aa 289-300.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSP90AA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:25000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1:500 - 1:2500
• Application Notes: IHC: NBP1-02459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSP90 alpha Antibody

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• HSP86
• Hsp89
• HSP89A
• HSP90 alpha
• Hsp90
• HSP90A
• HSP90AA1
• HSP90AHSP86
• HSP90N
• HSPC1
• HSPC1HSP 86
• HSPCA
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AA1
• Entrez:
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
  • NM_010480.5(Mouse)
• Uniprot:
  • P07190(Human)
  • P07190(Human)
  • P07190(Human)
  • P07190(Human)
  • P07190(Human)
  • P07190(Human)
  • P07190(Human)
  • P07901(Mouse)
  • P07901(Mouse)
  • P07901(Mouse)
  • P07901(Mouse)