HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

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Western Blot: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp40 monoclonal antibody (HepG2: human; ...read more
Immunocytochemistry/ Immunofluorescence: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp40.
Immunohistochemistry: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) Staining of paraffin-embedded Human prostate tissue using anti-Hsp40 mouse monoclonal antibody.
Flow Cytometry: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) ...read more
Western Blot: HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free [NBP2-70969] - Hsp40/DNAJB1 Antibody (1F9) HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hsp40 (Right lane) cDNA ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DNAJB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500-2000
Theoretical MW
37.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for HSP40/DNAJB1 Antibody (OTI1F9) - Azide and BSA Free

  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • DnaJ (Hsp40) homolog, subfmaily B, member 1
  • dnaJ homolog subfamily B member 1
  • DnaJ protein homolog 1
  • DNAJ1
  • DNAJB1
  • Hdj1
  • hDj-1
  • Heat shock 40 kDa protein 1
  • heat shock 40kD protein 1
  • Heat shock protein 40
  • HSP40
  • HSPF1
  • HSPF1Hdj1
  • Human DnaJ protein 1
  • radial spoke 16 homolog B
  • RSPH16B
  • Sis1

Background

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DNAJB1