HSP40/DNAJB1 Antibody (OTI1F9) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

HSP40/DNAJB1 Antibody (OTI1F9) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNAJB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HSP40/DNAJB1 Antibody (OTI1F9) [Alexa Fluor™ Plus 647]

• DnaJ (Hsp40) homolog, subfamily B, member 1
• DnaJ (Hsp40) homolog, subfmaily B, member 1
• dnaJ homolog subfamily B member 1
• DnaJ protein homolog 1
• DNAJ1
• DNAJB1
• Hdj1
• hDj-1
• Heat shock 40 kDa protein 1
• heat shock 40kD protein 1
• Heat shock protein 40
• HSP40
• HSPF1
• HSPF1Hdj1
• Human DnaJ protein 1
• radial spoke 16 homolog B
• RSPH16B
• Sis1

Background

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNAJB1