Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

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ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151] - A typical standard curve for HSP27 [p Ser15] ELISA kit. These standard curves are provided for demonstration only. A standard curve should be ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell lysates, plasma, and serum
Standard Curve Range
31.25 - 1000 pg/ml
Sensitivity
10.15 pg/ml

Order Details

Human HSP27 [p Ser15] ELISA Kit (Colorimetric) Summary

Modification
p Ser15
Specificity
Crossreactivity: Phopho HSP27 (Ser15) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and <0.016%: HSP25, HSP40, HSP90, alphaB Crystallin, alphaA Crystallin
Standard Curve Range
31.25 - 1000 pg/ml
Sensitivity
10.15 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
Low Concentration %CV - 9.9% Medium Concentration %CV - 9.6% High Concentration %CV - 7.1%
Intra-Assay
Low Concentration %CV - 14.2% Medium Concentration %CV - 9.3% High Concentration %CV - 4.0%
Spike Recovery
HeLa Cells (human) - 92%
Liver Microsomes - 103%
Serum (human) - 99%
EDTA Plasma - 103-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HSPB1

Applications/Dilutions

Dilutions
  • ELISA

Reactivity Notes

Crossreactivity: Phopho HSP27 (Ser15) (100%), HSP27 (non-phospho) (0.885%), HSP70 (0.023%) and <0.016%: HSP25, HSP40, HSP90, alphaB Crystallin, alphaA Crystallin

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 5X Extraction reagent
  2. Antibody
  3. Assay buffer 27
  4. Conjugate
  5. Microtiter plate
  6. Standard
  7. Stop solution 2
  8. TMB Substrate
  9. Wash buffer concentrate

Alternate Names for Human HSP27 [p Ser15] ELISA Kit (Colorimetric)

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HSPB1