HSP27 Antibody

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Genetic Strategies: Western Blot: HSP27 Antibody [NBP1-89666] - Analysis in human cell lines MCF-7 and Caco-2 using anti-HSPB1 antibody. Corresponding HSPB1 RNA-seq data are presented for the same cell lines. ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining in human skin and pancreas tissues using anti-HSPB1 antibody. Corresponding HSPB1 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human skin shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HSP27 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSS
Specificity
Specificity of human HSP27 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Positive Control
HSP27 Lysate (NBP2-64653)
Control Peptide
HSP27 Protein (NBP1-89666PEP)
Publications
Read Publication using NBP1-89666.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (83%), Rat (83%). Reactivity reported in scientific literature (PMID: 16524965)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSP27 Antibody

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-89666
Species: Hu
Applications: WB, IHC, IHC-P

Publications for HSP27 Antibody (NBP1-89666)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

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Bioinformatics

Gene Symbol HSPB1
Entrez