HSP10/EPF Antibody (JG82-34)

Western Blot: HSP10/EPF Antibody (JG82-34) [NBP2-75529] - Analysis of Cpn10 on mouse liver tissue lysate using anti-Cpn10 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: HSP10/EPF Antibody (JG82-34) [NBP2-75529] - Analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP10/EPF Antibody (JG82-34) [NBP2-75529] - Analysis of paraffin-embedded human liver cancer tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP10/EPF Antibody (JG82-34) [NBP2-75529] - Analysis of paraffin-embedded human colon tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP10/EPF Antibody (JG82-34) [NBP2-75529] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cpn10 antibody. Counter stained with hematoxylin.

Product Discontinued

HSP10/EPF Antibody (JG82-34) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HSP10/EPF aa 10-102 / 102. (SwissProt: P61604 Human; SwissProt: Q64433 Mouse; SwissProt: P26772 Rat)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSP10/EPF Antibody (JG82-34)

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

Hsp10 make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Also known as Cpn10, Hsp10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Hsp10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Hsp10 as a potential contributor to an immunological response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPE1