HSP10/EPF Antibody (4C11-B11) - Azide and BSA Free

Western Blot: HSP10/EPF Antibody (4C11-B11) [H00003336-M01] - Analysis of HSPE1 expression in transfected 293T cell line by HSPE1 monoclonal antibody (M01), clone 4C11-B11.Lane 1: HSPE1 transfected lysate(10.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HSP10/EPF Antibody (4C11-B11) [H00003336-M01] - Analysis of monoclonal antibody to HSPE1 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: HSP10/EPF Antibody (4C11-B11) [H00003336-M01] - HSPE1 monoclonal antibody (M01), clone 4C11-B11 Analysis of HSPE1 expression in COLO 320 HSR.

ELISA: HSP10/EPF Antibody (4C11-B11) [H00003336-M01] - Detection limit for recombinant GST tagged HSPE1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4C11-B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSP10/EPF Antibody (4C11-B11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HSPE1 (AAH23518, 1 a.a. ~ 102 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
• Specificity: HSPE1 - heat shock 10kDa protein 1 (chaperonin 10)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSP10/EPF Antibody (4C11-B11) - Azide and BSA Free

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP10/EPF Antibody (H00003336-M01)(1)

Publication using H00003336-M01

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: HSPE1
• Entrez:
  • 3336(Human)
• OMIM:
  • 600141(Human)
• Uniprot:
  • AAH23518(Human)