HSBP1 Antibody - BSA Free

Western Blot: HSBP1 Antibody - BSA Free [NBP3-46077] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (HSBP1 antibody) at dilution of 1:1000

Immunohistochemistry: HSBP1 Antibody - BSA Free [NBP3-46077] - Immunohistochemistry of paraffin-embedded human prostate cancer using (HSBP1 antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSBP1 Antibody - BSA Free (NBP3-46077) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: heat shock factor binding protein 1. (Uniprot# O75506)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HSBP1 Antibody - BSA Free

• DKFZp686D1664
• DKFZp686O24200
• heat shock factor binding protein 1
• heat shock factor-binding protein 1
• HSF1BP
• Nasopharyngeal carcinoma-associated antigen 13
• NPC-A-13

Background

The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation ofHSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor bindingprotein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factorinvolved in the HS response. During HS response, HSF1 undergoes conformational transition from an inertnon-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the activetrimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cellsrepresses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survivalof the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shockresponse. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSBP1