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HS3ST3A1 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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HS3ST3A1 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-HS3ST3A1 full-length
Gene
HS3ST3A1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for HS3ST3A1

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-HS3ST3A1 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HS3ST3A1 Overexpression Lysate (Denatured)

  • 3-OST-3A
  • 3OST3A1heparan sulfate glucosamine 3-O-sulfotransferase 3A1,30ST3A1heparin-glucosamine 3-O-sulfotransferase
  • EC 2.8.2
  • EC 2.8.2.30
  • h3-OST-3A
  • heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1
  • Heparan sulfate 3-O-sulfotransferase 3A1
  • Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1
  • HS3ST3A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Western Blot: HS3ST2 Antibody [NBP1-89374] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: HS3ST2 Antibody [NBP1-89374] - Staining of human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: HS3ST2 Antibody [NBP1-89374] - Staining of human duodenum shows distinct positivity in glandular cells.
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NBP2-23603
Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.
Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan‑1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3‑O‑Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
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Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
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Bioinformatics

Gene Symbol HS3ST3A1