Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Human HS3ST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HS3ST1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human HS3ST1
  Arg21-His307
  Accession # O14792
• Specificity: Detects human HS3ST1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HS3ST1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

• 3OST
• 3OST1
• 3-OST-1
• 3OST1Heparan sulfate 3-O-sulfotransferase 1
• EC 2.8.2
• EC 2.8.2.23
• h3-OST-1
• heparan sulfate (glucosamine) 3-O-sulfotransferase 1
• Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
• heparan sulfate glucosamine 3-O-sulfotransferase 1
• heparin-glucosamine 3-O-sulfotransferase
• HS3ST1

Background

Heparan sulfate is a highly sulfated polysaccharide that can be found on cell surface and within extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is detached from the protein core and is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑-acetylglucosamine residues can be sulfated at N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST1 is a rate-limiting enzyme for generating an antithrombin-binding pentasaccharide epitope on heparan sulfate and heparin (5, 6). Unlike other sulfotransferases that have signal-anchor domains and are type II membrane integral proteins in Golgi apparatus, HS3ST1 lacks a transmembrane domain and is likely to be an intraluminal enzyme (7, 8).

1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
4. Xu, D. et al. (2005) Biochem. J. 386:451.
5. Rosenberg, R.D. et al. (1997) J. Clin. Invest. 100:S67.
6. Esko, J.D and Lindahl, U. (2001) J. Clin. Invest. 108:169.
7. Shworak, N.W. et al. (1997) J. Biol. Chem. 272:28008.
8. Liu, J. et al. (1999) J. Biol. Chem. 274:5158.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HS3ST1
• Uniprot:
  • O14792()