| Reactivity | Hu, MuSpecies Glossary |
| Applications | IP |
| Clone | Y13-238 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rat HRAS Antibody (Y13-238) - Azide and BSA Free (NBP3-20119) is a recombinant monoclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21. |
| Specificity | The antibody reacts with v-HRAS (within amino acids 120-138) and rodent c-HRAS p21's but not v-KRAS or rodent c-KRAS p21's. This antibody also recognizes human c-HRAS and c-KRAS p21's, but not human c-NRAS. |
| Isotype | IgG2a Lambda |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | HRAS |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | HRAS |