HRAS Antibody (H-Ras-03) - BSA Free

Western Blot: vH RAS Antibody (H-Ras-03) [NB110-68799] - Western Blotting analysis (reducing conditions) of vH RAS Antibody (H-Ras-03) in whole cell lysate using anti-human vH RAS Antibody (H-Ras-03). Lane 1: HeLa human cervix carcinoma cell line Lane 2: K567 human leukemia cell line Lane 3: RAJI human Burkitt lymphoma cell line.

Analysis of human H-Ras using mouse monoclonal antibody H-Ras-03 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.

• Reactivity: Hu
• Applications: WB
• Clone: H-Ras-03
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HRAS Antibody (H-Ras-03) - BSA Free Summary

• Description: Novus Biologicals Mouse HRAS Antibody (H-Ras-03) - BSA Free (NB110-68799) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.
• Specificity: The antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HRAS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 -1 ug/ml
• Application Notes: Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Application note: Reducing conditions. Purity is > 95% (by SDS-PAGE).
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HRAS Antibody (H-Ras-03) - BSA Free

• C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Background

H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases vH RAS Antibody (H-Ras-03) affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of vH RAS Antibody (H-Ras-03) signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HRAS
• Entrez:
  • 3265(Human)
• Uniprot:
  • P01112(Human)