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Recombinant Human HPRT His Protein

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SDS-Page: HPRT Recombinant Protein [NBP2-53063] - 15% SDS-PAGE (3ug)
SDS-Page: HPRT Recombinant Protein [NBP2-53063] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
0.5 mg/ml

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Recombinant Human HPRT His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Human HPRT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
Details of Functionality
Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate(GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HPRT1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
26.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human HPRT His Protein

  • EC 2.4.2.8
  • HGPRT
  • HGPRT1
  • HGPRTHPRTHGPRTase
  • HPRT
  • HPRT1
  • hypoxanthine phosphoribosyltransferase 1
  • hypoxanthine-guanine phosphoribosyltransferase

Background

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody NB100-236 (lot 2) used for IP at 6 ug per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated G6PD, NB100-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p<0.05. Individual lanes indicate each animal (n?=?6). #p<0.05-between basal and EES. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY licence.
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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p53 Antibody (PAb
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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(34 Publications)
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NBP2-15291
Western Blot: ADK Antibody [NBP2-15291] - Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted by 1:1500.
Western Blot: ADK Antibody [NBP2-15291] - Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with Adenosine kinase antibody diluted by 1:1500.
Immunohistochemistry-Paraffin: ADK Antibody [NBP2-15291] - Mouse kidney. Adenosine kinase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

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Bioinformatics

Gene Symbol HPRT1