HPRT Antibody (OTI2F7) - Azide and BSA Free

Western Blot: HPRT Antibody (OTI2F7) - Azide and BSA Free [NBP2-70934] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT.

Immunocytochemistry/Immunofluorescence: HPRT Antibody (OTI2F7) - Azide and BSA Free [NBP2-70934] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HPRT.

Immunocytochemistry/Immunofluorescence: HPRT Antibody (OTI2F7) - Azide and BSA Free [NBP2-70934] - Analysis of HeLa cells.

Product Discontinued

HPRT Antibody (OTI2F7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human HPRT1(NP_000185) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HPRT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:6000
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for HPRT Antibody (OTI2F7) - Azide and BSA Free

• EC 2.4.2.8
• HGPRT
• HGPRT1
• HGPRTHPRTHGPRTase
• HPRT
• HPRT1
• hypoxanthine phosphoribosyltransferase 1
• hypoxanthine-guanine phosphoribosyltransferase

Background

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPRT1