HPRG Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HPRG Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HPRG
  Val19-Lys525
  Accession # P04196
• Specificity: Detects human HPRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse HPRG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HRG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HPRG Antibody [Unconjugated]

• DKFZp779H1622
• Histidine-proline-rich glycoprotein
• histidine-rich glycoprotein
• HPRG
• HPRGHRGP
• HRG

Background

Human histidine‑rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67‑75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N‑terminal cystatin‑like modules (aa 19‑254) and one His‑Pro‑rich region (aa 350‑497) that is flanked by two Pro‑rich segments (aa 276‑321 and 498‑525) (3, 4). The His‑Pro‑rich region contains 10 tandem repeats with an HHPHG motif, and the N‑ and C‑termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn⁺⁺‑dependent manner, it is converted to plasmin by tPA (7‑9). HPRG also shows antiangiogenic activity on endothelial cells (10).

1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
3. Koide, T. et al. (1986) Biochemistry 25:2220.
4. SwissProt Accession # P04196.
5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
7. Donate, F. et al. (2004) Cancer Res. 64:5812.
8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPRG Antibody (AF1869)(1)

Publication using AF1869

• H Kaihola, FG Yaldir, T Bohlin, R Samir, J Hreinsson, H Åkerud Levels of caspase-3 and histidine-rich glycoprotein in the embryo secretome as biomarkers of good-quality day-2 embryos and high-quality blastocysts PLoS ONE, 2019-12-19;14(12):e0226419. 2019-12-19 [PMID: 31856190] (Proximity Extension Assay, Human)

Bioinformatics

• Gene Symbol: HRG
• Uniprot:
  • P04196()