HPRG Antibody (227901) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 227901
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

HPRG Antibody (227901) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HPRG
  Val19-Lys525
  Accession # P04196
• Specificity: Detects human HPRG in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Cystatin B, C, D, E/M, S, A, SA, SN, rhFetuin A, B, rmHPRG, rhKininogen, or rhKininostatin.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HPRG Antibody (227901) [Alexa Fluor® 532]

• DKFZp779H1622
• Histidine-proline-rich glycoprotein
• histidine-rich glycoprotein
• HPRG
• HPRGHRGP
• HRG

Background

Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67 - 75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525) (3, 4). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn ++ -dependent manner, it is converted to plasmin by tPA (7 - 9). HPRG also shows antiangiogenic activity on endothelial cells (10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P04196()