HP1 alpha Antibody

Western Blot: HP1 alpha Antibody [NBP3-18650] - Figure 1. Western blot analysis using the antibody against hHP1 alpha Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against hHP1 alpha diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left. The location of the three HP1 isoforms of interest is indicated on the right, demonstrating that the antibody only recognizes the alpha isoform.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HP1 alpha Antibody Summary

• Description: Novus Biologicals Rabbit HP1 alpha Antibody (NBP3-18650) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human HP1 alpha (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBX5
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for HP1 alpha Antibody

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

Heterochromatin protein-1 (HP1) is a methyl-lysine binding adapter protein that is involved in a variety important functions in the cell nucleus. Localized at heterochromatin sites, HP1 is responsible for gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.

HP1 antibodies are useful tools for research on transcription regulation and epigenetic signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX5
• Uniprot:
  • P45973(Human)