HOXB4 Antibody (3A2F2) - BSA Free

Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against HEK293 (1) and HOXB4 (AA: 16-251)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HOXB4 mouse mAb with DAB staining.

Western Blot: HOXB4 Antibody (3A2F2) [NBP2-37257] - Western blot analysis using HOXB4 mAb against human HOXB4 (AA: 16-251) recombinant protein. (Expected MW is 51.9 kDa)

Immunohistochemistry-Paraffin: HOXB4 Antibody (3A2F2) [NBP2-37257] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HOXB4 mouse mAb with DAB staining.

ELISA: HOXB4 Antibody (3A2F2) [NBP2-37257] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunomicroscopy: HOXB4 Antibody (3A2F2) [NBP2-37257] - HeLa cells after 1 minute co-incubation using HoxB4 (7 micro M) and 6His-CM18-PTD4 (10 micro M) in fluorescent microscopy analysis. Prior to immune-labeling, cells were permeabilized and fixed with a primary mouse anti-HOXB4 monoclonal antibody (3A2F2). Scale Bars: 50 micro m. Citation: Del'Guidice T, Lepetit-Stoffaes J-P, Bordeleau L-J, Roberge J, Theberge V, Lauvaux C, et al. (2018) Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. PLoS ONE 13(4): e0195558. //doi.org/10.1371/journal.pone.0195558

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3A2F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HOXB4 Antibody (3A2F2) - BSA Free Summary

• Description: Novus Biologicals Mouse HOXB4 Antibody (3A2F2) - BSA Free (NBP2-37257) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-HOXB4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human HOXB4 (AA: 16-251) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOXB4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:100
  • Immunomicroscopy
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 27.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HOXB4 Antibody (3A2F2) - BSA Free

• homeo box 2F
• homeo box B4
• homeobox B4
• Homeobox protein Hox-2.6
• Homeobox protein Hox-2F
• homeobox protein Hox-B4
• HOX-2.6
• HOX2FHOX2

Background

Homebox transcription factors (Hox) are encoded by highly conserved developmental genes that are involved embryonic and early hematopoietic development. Specifically, Homeobox B4 (HoxB4) is a transcription factor encoded by HOXB4 gene and it is involved in the developmental regulation of specific positional identities on the anterior-posterior axis of cells. Like all members of HOX family, HoXB4 is abundantly expressed in primitive hematopoietic stem cell (HSC), but then decline with lineage-specific terminal differentiation (1). In HSC, stem cell self-renewal activity is regulated by USF1/2 binding to HoxB4, where binding possibly favors stem cell self-renewal instead of cell differentiation. Jun-B and Fra-1 has been linked as key mediators during cell proliferation and differentiation induced by HoxB4, which leads to increase expression of Cyclin D1 and G1 shortening (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXB4 Antibody (NBP2-37257)(1)

Publication using NBP2-37257

• Del'Guidice T, Lepetit-Stoffaes JP, Bordeleau LJ et al. Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. PLoS ONE 2018-04-04 [PMID: 29617431] (Human)

Bioinformatics

• Gene Symbol: HOXB4