Host Cell Factor 1/HCFC1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Host Cell Factor 1/HCFC1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Host Cell Factor 1/HCFC1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Host Cell Factor 1/HCFC1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Host Cell Factor 1/HCFC1
  Ala1626-Val1836
  Accession # P51610
• Specificity: Detects human Host Cell Factor 1/HCFC1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HCFC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Host Cell Factor 1/HCFC1 Antibody [Unconjugated]

• C1 Factor
• CFF
• CFFC1 factor
• HCF
• HCF1
• HCF1HFC1
• HCF-1VP16 accessory protein
• HCFC1
• HFC1
• host cell factor C1 (VP16-accessory protein)
• MGC70925
• VCAFhost cell factor 1

Background

HCF-1 (Host cell factor; also called C1 factor and VCAF) is a blanket name for a group of polypeptides that are generated through the targeted proteolysis of a large 300 kDa transcriptional coactivator precursor. The precursor is widely expressed, and its products are posited to participate in multiple activities, such as mRNA processing, transcriptional coactivation, and cell cycle progression through G0/G1 transition. Human HCF-1 is 2035 amino acids (aa) in length. It is modular in structure, and contains a Kelch-repeat region (aa 32-313), an SP1/GABP basic binding sequence (aa 478-875), an acidic transactivation domain (aa 1530-1735), and a C-terminal NLS-containing Trp/Tyr/Phe-rich region (aa 1760-2035). Situated between the basic and acidic regions is an HCF repeat region (aa 1010-1439) that contains six 26 aa repeats that undergo autocatalytic cleavage. HCF-1 is O-glycosylated, phosphorylated and acetylated. In quiescent cells, the full-length 300 kDa precursor can be found in the cytoplasm, along with a presumed 50 kDa fragment that appears to represent some sequence between aa 300-1000. In activated cells, HCF-1 appears in the nucleus, and undergoes autocatalysis at one or more sites occurs distal to Glu at position 1019, 1081, 1110, 1295, 1323, and 1423. This generates multiple fragments that noncovalently associate with each other and appear as bands in SDS-Page that range from 100-175 kDa in size. This association is quite vigorous and requires aggressive treatment to achieve denaturation. There is one potential isoform that contains an alternative start site at Met100, a second isoform that shows a deletion of aa 1072-1101, a third isoform that exhibits a Leu substitution for aa 428-2035, and a final isoform that shows a deletion of aa 382-450. Over aa 1626-1836, human HCF-1 shares 95% aa identity with mouse HCF-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Host Cell Factor 1/HCFC1 Antibody (AF6254)(1)

Publication using AF6254

• A Antonova, B Hummel, A Khavaran, DM Redhaber, F Aprile-Gar, P Rawat, K Gundel, M Schneck, EC Hansen, J Mitschke, G Mittler, C Miething, R Sawarkar Heat-Shock Protein 90 Controls the Expression of Cell-Cycle Genes by Stabilizing Metazoan-Specific Host-Cell Factor HCFC1 Cell Rep, 2019-11-05;29(6):1645-1659.e9. 2019-11-05 [PMID: 31693902] (Western Blot, Human)

Bioinformatics

• Gene Symbol: HCFC1
• Uniprot:
  • P51610(Human)