HOMER1 Antibody (666241) [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HOMER1 Monoclonal Antibody (Catalog # MAB6889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HOMER1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for HOMER1 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HOMER1 Monoclonal Antibody (Catalog # MAB6889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 666241
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HOMER1 Antibody (666241) [Unconjugated] Summary

• Immunogen: Synthetic peptide KFGQWADSRA corresponding to aa 73-82 of human HOMER1
  Accession # Q86YM7
• Specificity: Detects human HOMER1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HOMER1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HOMER1 Antibody (666241) [Unconjugated]

• homer homolog 1 (Drosophila)
• HOMER
• HOMER1
• homer-1
• HOMER1A
• HOMER1B
• HOMER-1B
• HOMER1C
• SYN47
• SYN47neuronal immediate early gene, 1
• Ves-1

Background

HOMER1 is an approximately 48 kDa cytoplasmic protein that regulates the cell surface localization of neuronal mGluR1 and mGluR5 metabotropic glutamate receptors. It also functions in striated muscle mechanotransduction and excitation-contraction coupling by regulating the localization of transient receptor potential (TRP) channels and the coupling of ryanodine receptors to Cav1.2 calcium channels. Alternately spliced isoforms of human HOMER1 lack either the C-terminal half of the molecule (aa 178-354) or 130 aa following the WH1 domain (aa 99-228). Particular isoforms show distinct effects on hippocampal neuronal plasticity and the maintenance of extracellular glutamate levels in the prefrontal cortex. Within aa 73-82, human HOMER1 shares 100% aa sequence identity with mouse and rat HOMER1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOMER1 Antibody (MAB6889)(3)

Publications using MAB6889

• Hendrik Demuth, Shirin Hosseini, Henning Peter Düsedeau, Ildiko Rita Dunay, Martin Korte, Marta Zagrebelsky Deletion of p75NTR rescues the synaptic but not the inflammatory status in the brain of a mouse model for Alzheimer’s disease Frontiers in Molecular Neuroscience 5/5/2023 [PMID: 37213691]
• MK Schroeder, B Liu, RG Hinshaw, MA Park, S Wang, S Dubey, GG Liu, Q Shi, P Holton, V Reiser, PA Jones, W Trigg, MF Di Carli, BJ Caldarone, JP Williams, MK O'Banion, CA Lemere Long-Term Sex- and Genotype-Specific Effects of 56Fe Irradiation on Wild-Type and APPswe/PS1dE9 Transgenic Mice International Journal of Molecular Sciences, 2021-12-10;22(24):. 2021-12-10 [PMID: 34948098] (Western Blot, Mouse, Transgenic Mouse)
• Henning Peter Düsedau, Johannes Steffen, Caio Andreeta Figueiredo, Julia Désirée Boehme, Kristin Schultz, Christian Erck et al. Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission mBio 10/27/2021 [PMID: 34700379]

Bioinformatics

• Gene Symbol: HOMER1
• Uniprot:
  • Q86YM7()