HO-2/HMOX2 Antibody

Western Blot: HO-2/HMOX2 Antibody [NBP1-36745] - Analysis of K562 cell lysate (35ug protein in RIPA buffer) using NBP1-36745 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HO-2/HMOX2 Antibody Summary

• Description: Novus Biologicals Goat HO-2/HMOX2 Antibody (NBP1-36745) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-HO-2/HMOX2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DQAGSTLARETLED corresponding to internal region according to NP_002125.3.
• Epitope: internal region
• Specificity: Reported variants represent identical protein: NP_002125.3, NP_001120676.1, NP_001120678.1, NP_001120677.1
• Predicted Species: Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMOX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in K562 lysates (calculated MW of 36.0 kDa band according to NP_002125.3). IHC-P: Human pancreas cancer shows increased staining of invading cancer cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HO-2/HMOX2 Antibody

• heme oxygenase (decycling) 2
• heme oxygenase 2
• HMOX2
• HO2
• HO-2
• HO-2EC 1.14.99.3

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-2/HMOX2 Antibody (NBP1-36745)(1)

Publication using NBP1-36745

• Sha L, Farrugia G, Harmsen WS, Szurszewski JH. Membrane potential gradient is carbon monoxide-dependent in mouse and human small intestine. Am J Physiol Cell Physiol 2007-08-01 [PMID: 17510199]

Bioinformatics

• Gene Symbol: HMOX2
• Entrez:
  • 3163(Human)
  • 15369(Mouse)
• Uniprot:
  • NP_002125.3(Human)