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hnRNP-Q Antibody (2E10)

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Sandwich ELISA: hnRNP-Q Antibody (2E10) [H00010492-M05] - Detection limit for recombinant GST tagged SYNCRIP is 0.1 ng/ml as a capture antibody.
Sandwich ELISA: hnRNP-Q Antibody (2E10) [H00010492-M05] - Detection limit for recombinant GST tagged SYNCRIP is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Clone
2E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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hnRNP-Q Antibody (2E10) Summary

Immunogen
SYNCRIP (AAH32643, 1 a.a. ~ 527 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATEHVNGNGTEEPMDTTSAVIHSENFQTLLDAGLPQKVAEKLDEIYVAGLVAHSDLDERAIEALKEFNEDGALAVLQQFKDSDLSHVQNKSAFLCGVMKTYRQREKQGTKVADSSKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGQQPSVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLNRGYAFVTFCTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPKSKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGFCFLEYEDHKTAAQVKVLFVRNLANTVTEEILEKAFSQFGKLERVKKLKDYAFIHFDERDGTVKAMEEMNGKDLEGENIEIVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRGGYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRGARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGQGKGVEAGPDLLQ
Specificity
Reacts with synaptotagmin binding, cytoplasmic RNA interacting protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SYNCRIP
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for hnRNP-Q Antibody (2E10)

  • dJ3J17.2
  • Glycine- and tyrosine-rich RNA-binding protein
  • GRYRBP
  • GRY-RBPNS1-associated protein 1
  • heterogeneous nuclear ribonucleoprotein Q
  • hnRNP Q
  • hnRNP-Q
  • HNRPQ
  • HNRPQ1
  • NSAP1FLJ31626
  • PP68
  • synaptotagmin binding, cytoplasmic RNA interacting protein
  • Synaptotagmin-binding, cytoplasmic RNA-interacting protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: hnRNP-R Antibody [NBP1-89676] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: hnRNP-R Antibody [NBP1-89676] - Staining in human endometrium and pancreas tissues using anti-HNRNPR antibody. Corresponding HNRNPR RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 1 ~ 10 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
ACF Antibody
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Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human colon shows moderate nuclear/cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Species: Hu
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GAPDH Antibody
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Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
(17 Reviews)
(326 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single-Cell Western
SMN Antibody (2B1)
NB100-1936
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (http://dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY licence.
Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.
Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
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NBP1-84929
Western Blot: EPRS Antibody [NBP1-84929] - Analysis using Anti-EPRS antibody NBP1-84929 (A) shows similar pattern to independent antibody NBP1-84930 (B).
Immunocytochemistry/Immunofluorescence: EPRS Antibody [NBP1-84929] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929] - Staining in human cerebral cortex and pancreas tissues using anti-EPRS antibody. Corresponding EPRS RNA-seq data are presented for the same tissues.
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
hnRNP C1 + C2
Antibody (SN0652)
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Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Species: Hu, Mu, Rt, Ze
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RPL13A Antibody
NBP1-32710
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Immunocytochemistry/Immunofluorescence: RPL13A Antibody [NBP1-32710] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RPL13A Antibody [NBP1-32710] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
HNRPDL Antibody
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Western Blot: HNRPDL Antibody [NBP1-57156] - Titration: 1.0ug/ml Positive Control: Daudi cell lysate.
Immunohistochemistry: HNRPDL Antibody [NBP1-57156] - Human cardiac cell Cellular data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.
Immunohistochemistry-Paraffin: HNRPDL Antibody [NBP1-57156] - Human Heart Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Myocardial cells (indicated with arrows) 400X magnification.
Species: Hu
Applications: WB, IHC, IHC-P
CSDE1 Antibody
NBP1-71915
Western Blot: CSDE1 Antibody [NBP1-71915] - Purification of Csde1 containing RNP complexes. MEL cells expressing BirA, or expressing BirA plus biotag-Csde1 were lysed, and incubated with streptavidin beads. Supernatant and beads were collected, beads were washed and eluted. Western blot with fractions was stained with anti-Csde1 antibody. Size markers are indicated in kDa. The positions of endogenous and biotagged Csde1 are indicated. A single empty lane has been cropped. The uncropped image is available as Supplementary Figure S5. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY licence.
Western Blot: CSDE1 Antibody [NBP1-71915] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNR antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. UNR was also immunoprecipitated by rabbit anti-UNR antibodies NBP1-71913 and NBP1-71914, which recognize upstream epitopes.
Knockdown Validated: CSDE1 Antibody [NBP1-71915] - Deletion of the 1st cold shock domain of Csde1 using Crispr/Cas9. Western blot stained for Csde1 and tubulin as loading control. The membrane was cut between Csde1 and tubulin and staining performed on each separately. D1 (2.1) and D2 (2.14) represent out-of-frame deletions (Del), H1 (2.121) and H2 (2.123) in-frame deletions of the 1st cold shock domain (Hm). HET are control clones that harbor 1 deleted (out-of-frame) allele and 1 wt allele. Raw image scans are available as Supplementary Figure S6. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY licence.
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APOBEC3B Antibody
NBP2-82073
Western Blot: APOBEC3B Antibody [NBP2-82073] - Analysis of APOBEC3B in HepG2 cell lysate with APOBEC3B antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: APOBEC3B Antibody [NBP2-82073] - Immunofluorescence of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 20 ug/mL.
Immunohistochemistry: APOBEC3B Antibody [NBP2-82073] - Immunohistochemistry of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 5 ug/mL.
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Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Citidine Deaminase
Antibody
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Western Blot: Citidine Deaminase Antibody [NBP2-39019] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue
Immunocytochemistry/Immunofluorescence: Citidine Deaminase Antibody [NBP2-39019] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Citidine Deaminase Antibody [NBP2-39019] - Staining of human cerebral cortex shows low expression as expected.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Stathmin 1 Antibody
NBP1-76798
Knockout Validated: Stathmin 1 Antibody [NBP1-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-76798] - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KO
SNRPN Antibody
NBP2-20439
Western Blot: SNRPN Antibody [NBP2-20439] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: SNRPN Antibody [NBP2-20439] - SNRPN antibody detects SNRPN protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPN antibody dilution: 1:500.
Western Blot: SNRPN Antibody [NBP2-20439] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
IP3R1 Antibody
NBP1-21398
Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, IF
hnRNP AB Antibody
NBP2-16865
Western Blot: hnRNP AB Antibody [NBP2-16865] - Wild-type (WT) and hnRNP AB knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP AB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: hnRNP AB Antibody [NBP2-16865] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A/B protein stained by hnRNP A/B antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: hnRNP AB Antibody [NBP2-16865] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Insulin Receptor
beta Antibody
NBP2-12793
Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500
Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
KHSRP Antibody
NBP1-18910
Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: KHSRP Antibody [NBP1-18910] - Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained for GS (green) and KHSRP (red). Image collected and cropped by CiteAb from the following publication (http://bmcbiol.biomedcentral.com/articles/10.1186/s12915-015-0137-1), licensed under a CC-BY licence.
Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

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Bioinformatics

Gene Symbol SYNCRIP
Entrez
Uniprot