hnRNP M Antibody (2A6)

Images

 
Western Blot: hnRNP M Antibody (2A6) [NB200-315] - Analysis of hnRNP M3-M4 in HeLa cell lysate.
Immunohistochemistry-Paraffin: hnRNP M Antibody (2A6) [NB200-315] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M (2A6) antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). ...read more
Simple Western: hnRNP M Antibody (2A6) [NB200-315] - Simple Western lane view shows a specific band for hnRNP M in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
2A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

hnRNP M Antibody (2A6) Summary

Immunogen
M19 fusion protein containing full-length human protein. [UniProt# P52272]
Specificity
NB200-315 is specific for hnRNP M3 and M4 proteins.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPM
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:3000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This hnRNP M (2A6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections, Immunoprecipitation and Western Blot. A 68 kDa band can be seen in a Western blot using HeLa cell lysate.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
hnRNP M Lysate (NBL1-11650)
Publications
Read Publications using
NB200-315 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for hnRNP M Antibody (2A6)

  • DKFZp547H118
  • heterogeneous nuclear ribonucleoprotein M
  • hnRNP M
  • HNRNPM4
  • HNRPM4heterogenous nuclear ribonucleoprotein M
  • HNRPMheterogenous nuclear ribonucleoprotein M4
  • HTGR1
  • M4 protein
  • N-acetylglucosamine receptor 1
  • NAGR1hnRNA-binding protein M4

Background

RNA-binding proteins of mammalian hnRNP (heterogeneous nuclear ribonucleo protein) family represents a group of over 20 distinct evolutionary conserved proteins which share a capability to bind pre-mRNA/mRNA, and also binds to specific RNA motifs with high-affinity. They exhibit huge functional diversity and implicates in almost every step in mRNA's life cycle (from transcription and processing to transport and translation). HnRNP M is an abundant protein containing three RNA recognition motifs (RRM), a glycine/methionine-rich region and a methionine/arginine-rich repeat motif. It has four protein variants, hnRNP M1-4, ranging in molecular size from 68 to 72 kD which corresponds to spliced isoforms and/or posttranslationally modified forms. hnRNP M is a nucleolar protein and has been Identified in the spliceosome C complex. It acts as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro, and is involved in splicing. hnRNP M acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and TNF-alpha cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for hnRNP M Antibody (NB200-315)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


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(1)
WB
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP M Antibody (NB200-315) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HNRNPM
Entrez
Uniprot