Western Blot: hnRNP M Antibody (2A6) [NB200-315] - Analysis of hnRNP M3-M4 in HeLa cell lysate.
Immunohistochemistry-Paraffin: hnRNP M Antibody (2A6) [NB200-315] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M (2A6) antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). ...read more
Simple Western: hnRNP M Antibody (2A6) [NB200-315] - Simple Western lane view shows a specific band for hnRNP M in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
This hnRNP M (2A6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections, Immunoprecipitation and Western Blot. A 68 kDa band can be seen in a Western blot using HeLa cell lysate.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Alternate Names for hnRNP M Antibody (2A6)
heterogeneous nuclear ribonucleoprotein M
HNRPM4heterogenous nuclear ribonucleoprotein M
HNRPMheterogenous nuclear ribonucleoprotein M4
N-acetylglucosamine receptor 1
NAGR1hnRNA-binding protein M4
RNA-binding proteins of mammalian hnRNP (heterogeneous nuclear ribonucleo protein) family represents a group of over 20 distinct evolutionary conserved proteins which share a capability to bind pre-mRNA/mRNA, and also binds to specific RNA motifs with high-affinity. They exhibit huge functional diversity and implicates in almost every step in mRNA's life cycle (from transcription and processing to transport and translation). HnRNP M is an abundant protein containing three RNA recognition motifs (RRM), a glycine/methionine-rich region and a methionine/arginine-rich repeat motif. It has four protein variants, hnRNP M1-4, ranging in molecular size from 68 to 72 kD which corresponds to spliced isoforms and/or posttranslationally modified forms. hnRNP M is a nucleolar protein and has been Identified in the spliceosome C complex. It acts as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro, and is involved in splicing. hnRNP M acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and TNF-alpha cytokines.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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