hnRNP K Antibody (F45 P9 C7) - BSA Free

Immunohistochemistry: hnRNP K Antibody (F45 P9 C7) [NBP3-30893] - Left and Center: Immunohistochemical staining of normal colon (left) and colon carcinoma (center) using hnRNP-K antibody (Cat. No. NBP3-30893).Right: Western blot using hnRNP-K antibody on Calu6 (1) and HT29 (2) cell lysates.

• Reactivity: Hu, Mu, Rt, Ch, Rb
• Applications: WB, IHC
• Clone: F45 P9 C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

hnRNP K Antibody (F45 P9 C7) - BSA Free Summary

• Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPK
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin : 1-5 ug/ml
  • Western Blot : 1-2 ug/ml
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for hnRNP K Antibody (F45 P9 C7) - BSA Free

• dC-stretch binding protein
• FLJ41122
• heterogeneous nuclear ribonucleoprotein K
• hnRNP K
• transformation upregulated nuclear protein
• Transformation up-regulated nuclear protein
• TUNPHNRPKCSBP

Background

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPK