hnRNP A1 Antibody (9H10) - BSA Free

Western Blot: hnRNP A1 Antibody (9H10) - Purified [NBP2-77365] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL of this antibody. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1) HEK-293T 2) HeLa 3) G361 4) COS7 5) MDBK 6) NRK 7) CHO

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody (9H10) - Purified [NBP2-77365] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 10 ug/mL of this antibody. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.

Product Discontinued

hnRNP A1 Antibody (9H10) - BSA Free Summary

• Immunogen: Purified human hnRNP-A1. Antigen mol wt ~34 kDa. Purified from hybridoma cell culture
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPA1
• Purity: Antigen and protein G Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Microarray
  • Western Blot 0.5-1.0 ug/mL
• Application Notes: Does not immunoprecipitate the hnRNP complex. hnRNP-A1 (heterogeneous nuclear ribonucleoprotein) participates in several RNA metabolisms. It also participates in deep vein thrombosis patients with behcet's disease. hnRNPA1 mutations results in amyotrophic lateral sclerosis and multisystem proteinopathy. WB: 0.5-1.0 ug/ml using total cell extract of 293T cells.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: Solution in 0.01 M phosphate buffered saline, pH 7.4
• Preservative: Sodium Azide
• Concentration: 2 mg/ml
• Purity: Antigen and protein G Affinity-purified from hybridoma culture supernatant

Alternate Names for hnRNP A1 Antibody (9H10) - BSA Free

• Helix-destabilizing protein
• heterogeneous nuclear ribonucleoprotein A1
• heterogeneous nuclear ribonucleoprotein A1B protein
• heterogeneous nuclear ribonucleoprotein B2 protein
• heterogeneous nuclear ribonucleoprotein core protein A1
• hnRNP A1
• hnRNP core protein A1
• hnRNPA1
• hnRNP-A1
• HNRPA1MGC102835
• nuclear ribonucleoprotein particle A1 protein
• single-strand DNA-binding protein UP1
• Single-strand RNA-binding protein

Background

hnRNP-A1 (heterogeneous nuclear ribonucleoprotein) is a pre-mRNA/mRNA binding protein, that belongs to the hnRNP family. It is located in chromosome 12q13.1. It is located in the nucleoplasm but it travels between the nucleus and the cytoplasm. It has two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPA1
• Entrez:
  • 15382(Mouse)
  • 29578(Rat)
• Uniprot:
  • P09651(Human)