HNF-3 beta/FoxA2 Antibody (1C7) - Azide and BSA Free

Western Blot: HNF-3 beta/FoxA2 Antibody (1C7) [H00003170-M10] - Analysis of FOXA2 expression in HepG2 (Cat # L019V1).

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (1C7) [H00003170-M10] - Analysis of monoclonal antibody to FOXA2 on HepG2 cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (1C7) [H00003170-M10] - Analysis of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml

ELISA: HNF-3 beta/FoxA2 Antibody (1C7) [H00003170-M10] - Detection limit for recombinant GST tagged FOXA2 is approximately 0.03ng/ml as a capture antibody.

Immunoprecipitation: HNF-3 beta/FoxA2 Antibody (1C7) [H00003170-M10] - Analysis of FOXA2 transfected lysate using anti-FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 1C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HNF-3 beta/FoxA2 Antibody (1C7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FOXA2 (NP_068556, 363 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
• Specificity: FOXA2 - forkhead box A2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HNF-3 beta/FoxA2 Antibody (1C7) - Azide and BSA Free

• forkhead box A2
• Forkhead box protein A2
• FoxA2
• hepatic nuclear factor-3-beta
• hepatocyte nuclear factor 3, beta
• hepatocyte nuclear factor 3-beta
• HNF3 beta
• HNF-3 beta
• HNF-3B
• HNF-3-beta
• HNF3BTCF-3B
• MGC19807
• TCF3B
• Transcription factor 3B

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 beta/FoxA2 Antibody (H00003170-M10)(5)

Publications using H00003170-M10

• Yu Y, Alonzo M, Ye S et al. Generation of an induced pluripotent stem cell line (NCHi010-A) from a 6-year-old female with Down syndrome and without congenital heart disease Stem Cell Research 2023-09-01 [PMID: 37392705]
• S Adhicary, S Ye, H Lin, K Texter, V Garg, MT Zhao Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation Stem Cell Research, 2022-12-31;66(0):103013. 2022-12-31 [PMID: 36599283]
• M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 2022-08-09;64(0):102892. 2022-08-09 [PMID: 35987121]
• J Contreras, M Alonzo, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Stem Cell Research, 2022-08-10;64(0):102893. 2022-08-10 [PMID: 35987120]
• J Kim, J Jeon, B Song, N Lee, S Ko, Y Cha, P Leblanc, H Seo, KS Kim Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells Nature Protocols, 2022-02-09;0(0):. 2022-02-09 [PMID: 35140411]

Bioinformatics

• Gene Symbol: FOXA2
• Entrez:
  • 3170(Human)
• OMIM:
  • 600288(Human)
• Uniprot:
  • NP_068556(Human)