HMGB3/HMG4 Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

HMGB3/HMG4 Antibody Summary

Immunogen
E. coli-derived recombinant human HMGB3
Met1-Val180
Accession # O15347
Specificity
Detects endogenous human/mouse HMGB3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HMGB3
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF5507 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGB3/HMG4 Antibody

  • High mobility group protein 2a
  • High mobility group protein 4
  • high mobility group protein B3
  • high-mobility group (nonhistone chromosomal) protein 4
  • high-mobility group box 3
  • HMG2A
  • HMG-2a
  • HMG2AHMG-4
  • HMG4
  • HMG4MGC90319
  • HMGB3
  • non-histone chromosomal protein

Background

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single-stranded DNA and unwinds double stranded DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for HMGB3/HMG4 Antibody (AF5507) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5507:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22912879
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22912879

Details

Detection NotesSee PMID 22912879

Comments

CommentsPublished: See PMID 22912879Independent review from antibodyresource.com

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Recent Reviews

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4
1
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1
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Anonymous
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol HMGB3
Uniprot