HMGB3/HMG4 Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5507) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HMGB3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HMGB3/HMG4 Antibody Summary

• Immunogen: E. coli-derived recombinant human HMGB3
  Met1-Val180
  Accession # O15347
• Specificity: Detects endogenous human/mouse HMGB3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMGB3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGB3/HMG4 Antibody

• High mobility group protein 2a
• High mobility group protein 4
• high mobility group protein B3
• high-mobility group (nonhistone chromosomal) protein 4
• high-mobility group box 3
• HMG2A
• HMG-2a
• HMG2AHMG-4
• HMG4
• HMG4MGC90319
• HMGB3
• non-histone chromosomal protein

Background

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single-stranded DNA and unwinds double stranded DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HMGB3/HMG4 Antibody (AF5507) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22912879
• Species: Mouse

Blocking

• Blocking Details: See PMID 22912879

Details

• Detection Notes: See PMID 22912879

Comments

• Comments: Published: See PMID 22912879Independent review from antibodyresource.com

Bioinformatics

• Gene Symbol: HMGB3
• Uniprot:
  • O15347()