HMG20A Antibody

Immunohistochemistry-Paraffin: HMG20A Antibody [NB300-860] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HMG20A Antibody Summary

• Description: Novus Biologicals Goat HMG20A Antibody (NB300-860) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-HMG20A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ATVREVVNRLDR corresponding to C-Terminus according to NP_060670.1.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HMG20A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB successful usage in rat brain is reported in scientific literature (PMID: 20530487). IHC-P: Human spleen shows staining of nucleoli.
• Control:

  HMG20A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HMG20A Antibody

• FLJ10739
• high-mobility group 20A
• HMG box domain containing 1
• HMG box-containing protein 20A
• HMG domain-containing protein 1
• HMGX1HMG domain-containing protein HMGX1
• HMGXB1high mobility group protein 20A

Background

HMG20A plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMG20A Antibody (NB300-860)(1)

Publication using NB300-860

• Artegiani B, Labbaye C, Sferra A et al. The interaction with HMG20a/b proteins suggests a potential role for beta-dystrobrevin in neuronal differentiation. J Biol Chem 2010-08-01 [PMID: 20530487]

Bioinformatics

• Gene Symbol: HMG20A
• Entrez:
  • 10363(Human)
  • 66867(Mouse)
• Uniprot:
  • NP_060670.(Human)