AP3S2 Antibody - Azide and BSA Free

Western Blot: AP3S2 Antibody [NBP3-15544] - Western blot analysis of extracts of various cell lines, using AP3S2 Rabbit pAb (NBP3-15544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: AP3S2 Antibody - Azide and BSA Free [NBP3-15544] - Immunofluorescence analysis of C6 cells using AP3S2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: AP3S2 Antibody - Azide and BSA Free [NBP3-15544] - Immunofluorescence analysis of U-2 OS cells using AP3S2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AP3S2 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit AP3S2 Antibody - Azide and BSA Free (NBP3-15544) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human AP3S2 (NP_005820.1). MIQAILVFNNHGKPRLVRFYQRFPEEIQQQIVRETFHLVLKRDDNICNFLEGGSLIGGSDYKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVHYILQEVVMGGMVLETNMNEIVAQIEAQNRLEKSEGGLSAAPARAVSAVKNINLPEIPRNINIGDLNIKVPNLSQFV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3S2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for AP3S2 Antibody - Azide and BSA Free

• Adapter-related protein complex 3 sigma-2 subunit
• adaptor complex sigma3B
• adaptor-related protein complex 3, sigma 2 subunit
• AP-3 complex subunit sigma-2
• AP-3 complex subunit sigma-3B
• AP3S3
• clathrin-associated/assembly/adaptor protein, small 4, 22-kD
• FLJ35955
• sigma3b
• sigma-3B-adaptin
• Sigma3B-adaptin
• Sigma-adaptin 3b

Background

Part of the AP-3 complex. an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: AP3S2