BRAF35 Antibody (1F6) - Azide and BSA Free

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - HMG20B monoclonal antibody (M01), clone IF6 Analysis of HMG20B expression in HeLa.

Immunocytochemistry/Immunofluorescence: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of monoclonal antibody to HMG20B on formalin-fixed paraffin-embedded human lung cancer. Antibody concentration 1.8 ug/ml.

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - Western blot analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody ( Cat # L013V1 ).

Western Blot: BRAF35 Antibody (1F6) [H00010362-M01] - Analysis of HMG20B in extracts from 293T, HeLa and A549 cell using anti-HMG20B monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BRAF35 Antibody (1F6) - Azide and BSA Free Summary

• Immunogen: HMG20B (NP_006330, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNV
• Specificity: HMG20B - high-mobility group 20B
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMG20B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for ELISA, immunofluoresence and immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BRAF35 Antibody (1F6) - Azide and BSA Free

• BRAF35Sox-like transcriptional factor
• high-mobility group 20B
• HMG domain-containing protein 2
• HMG domain-containing protein HMGX2
• HMGX2FLJ26127
• HMGXB2member 1-related
• SMARCE1R
• SMARCE1-related protein

Background

BRAF35 is required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRAF35 Antibody (H00010362-M01)(3)

Publications using H00010362-M01

• Zanchetta ME, Napolitano LMR, Maddalo D, Meroni G et al. The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35. Biochim Biophys Acta 2017-07-29 [PMID: 28760657]
• Lee M, Venkitaraman AR. A cancer-associated mutation inactivates a region of the high-mobility group protein HMG20b essential for cytokinesis. Cell Cycle 2014-01-01 [PMID: 25486196] (WB)
• Ceballos-Chavez M, Rivero S, Garcia-Gutierrez P et al. Control of neuronal differentiation by sumoylation of BRAF35, a subunit of the LSD1-CoREST histone demethylase complex. Proc Natl Acad Sci U S A. 2012-05-08 [PMID: 22570500]

Bioinformatics

• Gene Symbol: HMG20B
• Entrez:
  • 10362(Human)
• OMIM:
  • 605535(Human)
• Uniprot:
  • NP_006330(Human)