HMBS Antibody (3E8.)

Images

 
Western Blot: HMBS Antibody (3E8) [H00003145-M01] - Analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: ...read more
Western Blot: HMBS Antibody (3E8) [H00003145-M01] - analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.
Sandwich ELISA: HMBS Antibody (3E8) [H00003145-M01] - Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3E8.
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HMBS Antibody (3E8.) Summary

Immunogen
HMBS (AAH00520.1, 1 a.a. - 361 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKIPDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH
Specificity
HMBS (3E8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HMBS
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HMBS Antibody (3E8.)

  • hydroxymethylbilane synthasePre-uroporphyrinogen synthase
  • PBGDPBG-D
  • PORC
  • porphobilinogen deaminase
  • porphyria, acute; Chester type
  • UPSEC 2.5.1.61
  • uroporphyrinogen I synthase
  • uroporphyrinogen I synthetase

Background

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMBS
Entrez
OMIM
Uniprot