HLTF Antibody

Images

 
Western Blot: HLTF Antibody [NB100-280] - Nuclear extract (20 ug) from HeLa Cells. Antibody used at 0.5 ug/ml.
Western Blot: HLTF Antibody [NB100-280] - Detection of Human SMARCA3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCA3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HLTF Antibody Summary

Immunogen
A synthetic peptide, which represented a portion of human SMARCA3 encoded in part by exons 23 and 24 (LocusLink ID 6596).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLTF
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunoprecipitation 2 -10 ug/mg of lysate
Application Notes
Western blot (~115 kD band).
Positive Control
HeLa Lysate (NB800-PC9)
Publications
Read Publication using NB100-280.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HLTF Antibody

  • DNA-binding protein/plasminogen activator inhibitor 1 regulator
  • EC 3.6.1
  • EC 3.6.4.-
  • EC 6.3.2.-
  • helicase-like transcription factor
  • HIP116ARING finger protein 80
  • HIP116matrix associated, actin dependent regulator of chromatin
  • RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
  • subfamily a, member 3
  • Sucrose nonfermenting protein 2-like 3
  • ZBU1

Background

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as HLTF (helicase-like transcription factor) has been shown to bind DNA promoter elements and stimulate transcription in cooperation with Sp1and Sp3 transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLTF Antibody (NB100-280)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLTF