Recombinant Human HLF His Protein

SDS-Page: Recombinant Human HLF Protein [NBP1-72413] - 15% SDS-PAGE (3ug)

Product Discontinued

Recombinant Human HLF His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-295 of Human HLF
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HLF
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 35.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.2 M NaCl, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human HLF His Protein

• hepatic leukemia factor
• MGC33822

Background

HLF, also known as hepatic leukemia factor, belongs to the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins. HLF binds DNA specifically as a homodimer or heterodimer with other PAR factors. It is highly expressed in liver, with lower levels in lung and kidney and functions as a potent trans-activator. Recombinant human HLF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HLF