HLA-DR Antibody (LN-3) - Azide and BSA Free

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Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3) - Azide and BSA Free [NBP2-47670] - Analysis using Azide and BSA Free version of NBP2-47670. Formalin-paraffin human tonsil stained with HLA-DR MAb (LN-3).

Product Details

Summary
Reactivity Hu, Mk, Mu(-)Species Glossary
Applications WB, Flow, IHC-P, CyTOF-ready, ICC/IF
Clone
LN-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

HLA-DR Antibody (LN-3) - Azide and BSA Free Summary

Immunogen
Activated human peripheral blood mononuclear cells
Localization
Cell Surface
Specificity
This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DRA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot : 0.5 - 1.0 ug/ml
  • Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml
  • CyTOF-ready
  • Immunofluorescence : 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 6p21.3 Molecular weight of antigen: 28kDa (beta chain) Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for HLA-DR Antibody (LN-3) - Azide and BSA Free

  • FLJ51114
  • histocompatibility antigen HLA-DR alpha
  • HLA class II histocompatibility antigen, DR alpha chain
  • HLADR
  • HLA-DR
  • HLA-DRA
  • HLA-DRA1
  • major histocompatibility complex, class II, DR alpha
  • MHC cell surface glycoprotein
  • MHC class II antigen DRA
  • MHC Class II DR
  • MLRW

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HLA-DRA
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