HLA-DR Antibody (L203) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) or (B) isotype control antibody (Catalog # IC002P) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Costimulatory profile of mature DCs generated with a 3-day protocol. (A) Shown is the expression of specific surface molecules detected on DCs by flow cytometry in a representative patient sample generated with cocktail RP. (B) Comparison of the expression intensity of the costimulatory molecules CD80 and CD86 and the coinhibitory molecules CD273 and CD274 between HCs and AML patients. The mean fluorescence (MFI) of at least three individual experiments of HC and AML patients were compared using the different maturation cocktails C, CP, R, and RP. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-9-151), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: L203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

HLA-DR Antibody (L203) [Phycoerythrin] Summary

• Immunogen: RPMI 8866 human lymphoblastoid cells
  Accession # P01903
• Specificity: Detects human HLA‑DR.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA-DR Antibody (L203) [Phycoerythrin]

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLADR
• HLA-DR
• HLA-DRA
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MHC Class II DR
• MLRW

Background

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA-DR Antibody (FAB4869P)(11)

Publications using FAB4869P

• F Li, C Gao, X Li, J Wang, Y Zhao, Y Ke, Y Liu, HM Liu, Z Hu, L Wei, ZS Chen Jiyuan oridonin A induces differentiation of acute myeloid leukemia cells including leukemic stem-like cells Frontiers in Pharmacology, 2022-09-05;13(0):1001552. 2022-09-05 [PMID: 36133825] (Flow Cytometry, Human)
• Iris A. Otto, Paulina Nuñez Bernal, Margot Rikkers, Mattie H.P. van Rijen, Anneloes Mensinga, Moshe Kon et al. Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential iScience 2022-09-16 [PMID: 36105583]
• Jingfang Yao, Gentao Li, Zihui Cui, Peilei Chen, Jinhong Wang, Zhenbo Hu et al. The Histone Deacetylase Inhibitor I1 Induces Differentiation of Acute Leukemia Cells With MLL Gene Rearrangements via Epigenetic Modification Frontiers in Pharmacology 2022-04-27 [PMID: 35571127]
• Xiangyu Ma, Mengjie Zhao, Zhuo-Xun Wu, Jingfang Yao, Lei Zhang, Jinhong Wang et al. The Histone Deacetylase Inhibitor I13 Induces Differentiation of M2, M3 and M5 Subtypes of Acute Myeloid Leukemia Cells and Leukemic Stem-Like Cells Frontiers in Oncology 2022-04-12 [PMID: 35494054]
• Andrew Kozbial, Lekhana Bhandary, Shashi K. Murthy Effect of monocyte seeding density on dendritic cell generation in an automated perfusion-based culture system Biochemical Engineering Journal 2019-10-15 [PMID: 32831621]
• S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial Biomed Res Int, 2020-07-27;2020(0):8748934. 2020-07-27 [PMID: 32802883] (Flow Cytometry, Human)
• Y Wu, Y Yan, Z Su, Q Bie, X Chen, PA Barnie, Q Guo, S Wang, H Xu Enhanced circulating ILC2s and MDSCs may contribute to ensure maintenance of Th2 predominant in patients with lung cancer Mol Med Rep, 2017-05-02;15(6):4374-4381. 5/2/2017 [PMID: 28487978]
• Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition J Clin Invest, 2016-07-25;126(8):3130-44. 2016-07-25 [PMID: 27454297] (Flow Cytometry, Human)
• Michael E. Yeager, Cecilia M. Nguyen, Dmitry D. Belchenko, Kelley L. Colvin, Shinichi Takatsuki, D. Dunbar Ivy et al. Circulating Myeloid-Derived Suppressor Cells Are Increased and Activated in Pulmonary Hypertension Chest 2012-04-01 [PMID: 21940769]
• Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission. J Transl Med, 2011-09-13;9(0):151. 2011-09-13 [PMID: 21910911] (Flow Cytometry, Human)
• Lotfi R, Lotze MT Eosinophils induce DC maturation, regulating immunity. J. Leukoc. Biol., 2007-11-08;83(3):456-60. 2007-11-08 [PMID: 17991762] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: HLA-DRA
• Uniprot:
  • P01903()