HLA-DR Antibody (1083110) [Unconjugated]

HLA-DRA was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 1 µg/mL at 37 ° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​

Western Blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA‑DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

HLA‑DR was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 1 mg/ml. A specific band was detected for HLA‑DR at approximately 42 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HLA‑DR Monoclonal Antibody (Catalog # MAB11555). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, mIF
• Clone: 1083110
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HLA-DR Antibody (1083110) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # P01903
• Specificity: Detects a synthetic peptide around aa 100 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Multiplex Immunofluorescence 1 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HLA-DR Antibody (1083110) [Unconjugated]

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLADR
• HLA-DR
• HLA-DRA
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MHC Class II DR
• MLRW

Background

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01903()