HLA DPB1 Antibody (HLA-DPB1/2862R)

Images

 
Immunocytochemistry/ Immunofluorescence: HLA DPB1 Antibody (HLA-DPB1/2862R) [NBP3-07722] - Immunofluorescence Analysis of Human Raji cells labeling HLA DPB1 with HLA DPB1 Rabbit Recombinant Monoclonal (HLA DPB1B1/2862R) ...read more
Flow Cytometry: HLA DPB1 Antibody (HLA-DPB1/2862R) [NBP3-07722] - Flow Cytometric Analysis of Raji cells HLA DPB1 Rabbit Recombinant Monoclonal Antibody (HLA DPB1B1/2862R) followed by goat anti-Rabbit IgG-CF488 (Blue); ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF
Clone
HLA-DPB1/2862R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HLA DPB1 Antibody (HLA-DPB1/2862R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
Localization
Cell Surface
Specificity
Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HLA-DPB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
Application Notes
ELISA: For coating use Ab at 2-4ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08719)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for HLA DPB1 Antibody (HLA-DPB1/2862R)

  • DP beta 1 chain
  • DPB1
  • HLA class II histocompatibility antigen, DP(W4) beta chain
  • HLA DP14-beta chain
  • HLA-DP histocompatibility type, beta-1 subunit
  • HLA-DP1B
  • HLA-DPB
  • major histocompatibility complex class II HLA DPB1 protein
  • major histocompatibility complex, class II, DP beta 1
  • MHC class II antigen beta chain
  • MHC class II antigen DP beta 1 chain
  • MHC class II antigen DPB1
  • MHC class II antigen DPbeta1
  • MHC class II HLA-DP-beta
  • MHC HLA DPB1

Background

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HLA-DPB1
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