HLA DPB1 Antibody (HLA-DPB1/2862R) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: HLA-DPB1/2862R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 594

HLA DPB1 Antibody (HLA-DPB1/2862R) [Alexa Fluor® 594] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Localization: Cell Surface
• Specificity: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HLA-DPB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HLA DPB1 Antibody (HLA-DPB1/2862R) [Alexa Fluor® 594]

• DP beta 1 chain
• DPB1
• HLA class II histocompatibility antigen, DP(W4) beta chain
• HLA DP14-beta chain
• HLA-DP histocompatibility type, beta-1 subunit
• HLA-DP1B
• HLA-DPB
• major histocompatibility complex class II HLA DPB1 protein
• major histocompatibility complex, class II, DP beta 1
• MHC class II antigen beta chain
• MHC class II antigen DP beta 1 chain
• MHC class II antigen DPB1
• MHC class II antigen DPbeta1
• MHC class II HLA-DP-beta
• MHC HLA DPB1

Background

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DPB1