HLA DMA Antibody (6B12NB) - Azide Free

Images

 
Western Blot: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA ...read more
Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody ...read more
Flow (Cell Surface): HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Human PBMC cells were stained (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow-CS, ICC/IF, IHC, IHC-P
Clone
6B12NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide Free

Order Details

HLA DMA Antibody (6B12NB) - Azide Free Summary

Immunogen
Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]
Localization
Membranes of Late Endosomes / Lysosomes
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DMA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface) 2.5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for HLA DMA Antibody (6B12NB) - Azide Free

  • class II histocompatibility antigen, M alpha chain
  • D6S222E
  • DMA
  • HLA class II histocompatibility antigen, DM alpha chain
  • major histocompatibility complex, class II, DM alpha
  • MHC class II antigen DMA
  • Really interesting new gene 6 protein
  • RING6HLADM

Background

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-DMA