HLA DMA Antibody (6B12NB) - Azide Free

Western Blot: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB) Image using the standard format of this product.

Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HLA DMA (6B12NB) at a 1:40 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 6B12NB). The antibody generated a punctate staining in the cytoplasm/perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein. Image using the standard format of this product.

Flow (Cell Surface): HLA DMA Antibody (6B12NB) - Azide Free [NBP2-44304] - Human PBMC cells were stained (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.

• Reactivity: Hu, Pm
• Applications: WB, Flow-CS, ICC/IF, IHC, IHC-P
• Clone: 6B12NB
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

HLA DMA Antibody (6B12NB) - Azide Free Summary

• Immunogen: Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]
• Localization: Membranes of Late Endosomes / Lysosomes
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DMA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 2.5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for HLA DMA Antibody (6B12NB) - Azide Free

• class II histocompatibility antigen, M alpha chain
• D6S222E
• DMA
• HLA class II histocompatibility antigen, DM alpha chain
• major histocompatibility complex, class II, DM alpha
• MHC class II antigen DMA
• Really interesting new gene 6 protein
• RING6HLADM

Background

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DMA