HLA DMA Antibody (6B12NB)

Images

 
Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody ...read more
Immunocytochemistry/ Immunofluorescence: HLA DMA Antibody (6B12NB) [NBP2-44302] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) [NBP2-44302] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone ...read more
Flow (Cell Surface): HLA DMA Antibody (6B12NB) [NBP2-44302] - Analysis using the Azide Free version of NBP2-44302. Detection of Human PBMC cells (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow-CS, ICC/IF, IHC, IHC-P
Clone
6B12NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HLA DMA Antibody (6B12NB) Summary

Immunogen
Partial recombinant human HLA DMA protein (between amino acids 10-300) [Entrez NP_006111]
Localization
Membranes of Late Endosomes / Lysosomes
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DMA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Cell Surface) 2.5 ug / million cells

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HLA DMA Antibody (6B12NB)

  • class II histocompatibility antigen, M alpha chain
  • D6S222E
  • DMA
  • HLA class II histocompatibility antigen, DM alpha chain
  • major histocompatibility complex, class II, DM alpha
  • MHC class II antigen DMA
  • Really interesting new gene 6 protein
  • RING6HLADM

Background

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA DMA Antibody (NBP2-44302) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-44302AF350
Alexa Fluor 405 NBP2-44302AF405
Alexa Fluor 488 NBP2-44302AF488
Alexa Fluor 532 NBP2-44302AF532
Alexa Fluor 594 NBP2-44302AF594
Alexa Fluor 647 NBP2-44302AF647
Alexa Fluor 700 NBP2-44302AF700
Alexa Fluor 750 NBP2-44302AF750
Allophycocyanin NBP2-44302APC
Biotin NBP2-44302B
DyLight 350 NBP2-44302UV
DyLight 405 NBP2-44302V
DyLight 488 NBP2-44302G
DyLight 550 NBP2-44302R
DyLight 594 NBP2-44302DL594
DyLight 650 NBP2-44302C
DyLight 680 NBP2-44302FR
DyLight 755 NBP2-44302IR
FITC NBP2-44302F
HRP NBP2-44302H
Janelia Fluor 549 NBP2-44302JF549
Janelia Fluor 646 NBP2-44302JF646
PE NBP2-44302PE
PerCP NBP2-44302PCP

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Bioinformatics

Gene Symbol HLA-DMA
Entrez
Uniprot