HLA B Antibody (EP-4)

• Reactivity: Hu
• Applications: Flow, ICC/IF, ICC/IF
• Clone: EP-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HLA B Antibody (EP-4) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11439)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Lymphocytes from an HLA-B27 patient
• Localization: Cell Surface
• Specificity: This monoclonal antibody recognizes the HLA-B27 cell surface antigen on human cells. It may be used to HLA type human lymphocytes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind pep- tides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain, which hetero-dimerizes with a light chain (-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-B
• Purity: Protein L or PEG purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein L or PEG purified

Alternate Names for HLA B Antibody (EP-4)

• ankylosing spondylitis
• AS
• B-4901
• b'DT
• Bw-22
• Bw-4
• Bw-41
• Bw-44
• Bw-45
• Bw-46
• Bw-47
• Bw-48
• Bw-50
• Bw-52
• Bw-53
• Bw-54
• Bw-55
• Bw-56
• Bw-57
• Bw-58
• Bw-60
• HLA class I histocompatibility antigen, B alpha chain
• HLA class I histocompatibility antigen, B-12 alpha chain
• HLA class I histocompatibility antigen, B-21 alpha chain
• HLA class I histocompatibility antigen, B-5 alpha chain
• HLA class I histocompatibility antigen, B-7 alpha chain
• HLAB
• HLA-B
• HLA-B27
• HLA-B73
• leukocyte antigen class I-B
• lymphocyte antigen
• major histocompatibility complex, class I, B
• MGC111087
• MHC class I antigen B*13
• MHC class I antigen B*14
• MHC class I antigen B*15
• MHC class I antigen B*18
• MHC class I antigen B*27
• MHC class I antigen B*35
• MHC class I antigen B*37
• MHC class I antigen B*38
• MHC class I antigen B*39
• MHC class I antigen B*40
• MHC class I antigen B*41
• MHC class I antigen B*42
• MHC class I antigen B*44
• MHC class I antigen B*45
• MHC class I antigen B*46
• MHC class I antigen B*47
• MHC class I antigen B*48
• MHC class I antigen B*49
• MHC class I antigen B*50
• MHC class I antigen B*51
• MHC class I antigen B*52
• MHC class I antigen B*53
• MHC class I antigen B*54
• MHC class I antigen B*55
• MHC class I antigen B*56
• MHC class I antigen B*57
• MHC class I antigen B*58
• MHC class I antigen B*59
• MHC class I antigen B*67
• MHC class I antigen B*7
• MHC class I antigen B*73
• MHC class I antigen B*78
• MHC class I antigen B*8
• MHC class I antigen B*81
• MHC class I antigen B*82
• MHC class I antigen GN00104
• MHC class I antigen HLA-B heavy chain
• MHC class I antigen SHCHA
• MHC Class I HLA heavy chain
• MHC HLA-B cell surface glycoprotein
• SPDA1

Background

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-B
• Uniprot:
  • P03989(Human)