Histone H4 [ac Lys8] Antibody (GT478) - Azide and BSA Free

Western Blot: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys8) antibody (NBP3-13539) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:400. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, ICC/IF, IHC
• Clone: GT478
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone H4 [ac Lys8] Antibody (GT478) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.
• Modification: ac Lys8
• Predicted Species: Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: H4C16
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Histone H4 [ac Lys8] Antibody (GT478) - Azide and BSA Free

• H4
• H4/A
• H4/B
• H4/C
• H4/D
• H4/E
• H4/G
• H4/H
• H4/I
• H4/J
• H4/K
• H4/M
• H4/N
• H4/O
• H4/p
• H4F2
• H4FA
• H4FB
• H4FC
• H4FD
• H4FE
• H4FG
• H4FH
• H4FI
• H4FJ
• H4FK
• H4FM
• H4FN
• H4FO
• H4M
• HIST1H4A
• HIST1H4B
• HIST1H4C
• HIST1H4D
• HIST1H4E
• HIST1H4F
• HIST1H4H
• HIST1H4I
• HIST1H4J
• HIST1H4K
• HIST1H4L
• HIST2H4
• HIST2H4A
• HIST2H4B
• HIST4H4
• histone 4, H4
• Histone Cluster 1 H4
• Histone Cluster 1 H4i
• Histone Cluster 4 H4
• histone cluster 4, H4
• Histone H4
• MGC24116

Background

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H4C16