Histone H4 [Dimethyl Lys20] Antibody (282) - Azide and BSA Free

Western Blot: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [282] dilution: 1:5000

Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [282] diluted at 1:500. Blue: Hoechst 33342 staining.

Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody (282) [NBP2-42860] - Analysis of HIST1H4A protein from HeLa whole cell extracts using 5 ug of Histone H4K20me2 (dimethyl Lys20) antibody [282]. Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [282]. EasyBlot anti-mouse IgG was used as a secondary reagent.

• Reactivity: Hu, Ye
• Applications: WB, DB, ICC/IF, IP, ChIP
• Clone: 282
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone H4 [Dimethyl Lys20] Antibody (282) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Histone H4 [Dimethyl Lys20] Antibody (282) - Azide and BSA Free (NBP2-42860) is a monoclonal antibody validated for use in WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys20 of human Histone H4. The exact sequence is proprietary.
• Modification: Dimethyl Lys20
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: H4C16
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Dot Blot 1:500
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Application Notes: Chip-Assay dependent
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Histone H4 [Dimethyl Lys20] Antibody (282) - Azide and BSA Free

• H4
• H4/A
• H4/B
• H4/C
• H4/D
• H4/E
• H4/G
• H4/H
• H4/I
• H4/J
• H4/K
• H4/M
• H4/N
• H4/O
• H4/p
• H4F2
• H4FA
• H4FB
• H4FC
• H4FD
• H4FE
• H4FG
• H4FH
• H4FI
• H4FJ
• H4FK
• H4FM
• H4FN
• H4FO
• H4K20Me2
• H4M
• HIST1H4A
• HIST1H4B
• HIST1H4C
• HIST1H4D
• HIST1H4E
• HIST1H4F
• HIST1H4H
• HIST1H4I
• HIST1H4J
• HIST1H4K
• HIST1H4L
• HIST2H4
• HIST2H4A
• HIST2H4B
• HIST4H4
• histone 4, H4
• Histone Cluster 1 H4
• Histone Cluster 1 H4i
• Histone Cluster 4 H4
• histone cluster 4, H4
• Histone H4
• MGC24116

Background

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H4C16