Histone H3 [Trimethyl Lys56] Antibody

Images

 
Western Blot: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - WB analysis of Histone H3K56me3 in C. elegans embryo lysate.
Immunocytochemistry: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Immunofluorescence: [NB21-1343] - Histone H3 K56me3 antibody was tested in HeLa cells during telophase with Dylight 488 (green). Nuclei and ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Histone H3 K56me3 antibody was tested in HeLa cells during prophase with Dylight 488 (green). Nuclei and alpha-tubulin were ...read more
Dot Blot: Histone H3 [Trimethyl Lys56] Antibody [NB21-1343] - Dot Blot analysis of H3K56me3 using the peptides stated above in 100, 10 and 1 picomoles of peptide.

Product Details

Summary
Reactivity Hu, Mu, Ce, Chicken, Drosophila, Plant, Rat, XenopusSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Concentration
0.71 mg/ml

Histone H3 [Trimethyl Lys56] Antibody Summary

Immunogen
Synthetic trimethylated peptide surrounding Lysine 56 of human Histone H3 [Swiss Prot Q71DI3].
Modification
Trimethyl Lys56
Localization
Nucleus. Chromosome.
Predicted Species
Rat (100%), Chicken (100%), Plant (100%), Drosophila (100%), Xenopus (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.71 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:40
Application Notes
This Histone H3 K56me3 antibody is useful for Dot Blot, Western Blot, ChIP*, and Immunocytochemistry/Immunofluorescence. In Western Blot, a band was seen ~15 kDa in C. elegans embryo lysate. In ICC/IF, staining was observed in the nucleus during mitosis in HeLa cells.

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Notes

* Chromatin immunoprecipitation was performed on this product with a standard set of primers that amplify active gene loci (GAPDH, RPL30), inactive loci (MYOD1, AFM), and heterochromatic loci (alphaSat, SAT2). The resulting data indicated there was little to no amplification of these sites, which corresponds with the current literature. Until a more specific locus can be established and validated, we give this product validity for ChIP and it is backed by the full Novus guarantee.
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [Trimethyl Lys56] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Trimethylation of lysine 56 on H3 is a novel modification, but has been reported in mouse cells at very low frequencies (PMID 14690609).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1343). (Showing 1 - 1 of 1 FAQ).

  1. We recently bought the anti-Histone H3 [Trimethyl Lys56] antibody (# NB21-1343, lot A-1). Our application would be ChIP and amino acid context of H3K56 is quite similar to H3K9, so the absence of cross reactivity with H3K9me3 (H3 [Trimethyl Lys9] ) is crucial. Do you have a dot blot assessing the specificity/sensitivity of the antibody?
    • While we haven't directly tested for this potential cross-reactivity, alignment of the immunogen with Histone H3 suggests that chances of cross-reactivity with H3K9 are extremely low. We feel quite confident that this antibody is specific for H3K56.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1343AF405
Alexa Fluor 488 Labeled NB21-1343AF488
Alexa Fluor 647 Labeled NB21-1343AF647
Alexa Fluor 700 Labeled NB21-1343AF700
Biotin Labeled NB21-1343B
DyLight 405 Labeled NB21-1343V
DyLight 488 Labeled NB21-1343G
DyLight 550 Labeled NB21-1343R
DyLight 650 Labeled NB21-1343C
FITC Labeled NB21-1343F
HRP Labeled NB21-1343H

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Blogs on Histone H3.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot

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