Histone H3 [p Thr6, Monomethyl Lys9] Antibody

Images

 
Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Histone H3 [Monomethyl Lys9, p Thr6] Antibody [NB21-1052] - WB analysis of H3K9me1/pT6 in NIH 3T3 Histone Prep lysate.
Immunocytochemistry: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Immunofluorescence: [NB21-1052] - Histone H3.2 K9me1 pT6 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were ...read more
Chromatin Immunoprecipitation: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1052 and 20ul of magnetic IgG beads ...read more
Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Histone H3 [Monomethyl Lys9, p Thr6] Antibody [NB21-1052] - WB analysis of H3K9me1/pT6 in C. elegans embryo lysate.
Western Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Histone H3 [Monomethyl Lys9, p Thr6] Antibody [NB21-1052] - WB analysis of H3K9me1/pT6 in HeLa Histone prep lysate.
Dot Blot: Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052] - Histone H3 [Monomethyl Lys9, p Thr6] Antibody [NB21-1052] - Dot blot analysis of H3K9me1/pT6 in picomoles of protein.
Histone H3 [p Thr6, Monomethyl Lys9] Antibody [NB21-1052]

Product Details

Summary
Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.85 mg/ml

Histone H3 [p Thr6, Monomethyl Lys9] Antibody Summary

Immunogen
Synthetic monomethylated/phosphorylated peptide surrounding Lysine 9 / Threonine 6 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
p Thr6, Monomethyl Lys9
Localization
Nucleus. Chromosome.
Predicted Species
Rat (100%), Xenopus (100%), Drosophila (100%), Plant (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes
This Histone H3K9me1/pT6 antibody is useful for ChIP, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15 kDa in HeLa histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate.
Positive Control
HeLa Lysate (NBP1-78155)
NIH 3T3 Lysate (NBP1-78157)

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.85 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3 [p Thr6, Monomethyl Lys9] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K9Me1
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Methylation of Histone H3 at Lys9 (K9) is an epigenetic silencer of transcription. Gene silencing from histone post translational modifications, as well as DNA methylation, play a key role in the development of normal tissues. If this silencing is disturbed through the artificial silencing of RIZ1, and thereby H3 K9Me1, it has been shown that normal apoptotic processes in precancerous cells can be reduced. Interestingly, data indicates that the conversion of the monomethyl to the trimethyl form requires mediation by SUVR4 in transposons and pseudogenes. Research also indicates that the presence of the G9a/GLP heterodimeric complex is required for this modification to exist. The additional phosphorylation at Thr6 (pT6) affects the ability of other proteins to bind to the H3 tail, along with amplifing the effects of other histone PTMs that are present. Because T6 phosphorylation is constitutive, its dephosphorylation may play a key role in DNA transcription, repair and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1052) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1052AF405
Alexa Fluor 488 Labeled NB21-1052AF488
Alexa Fluor 647 Labeled NB21-1052AF647
Alexa Fluor 700 Labeled NB21-1052AF700
Biotin Labeled NB21-1052B
DyLight 405 Labeled NB21-1052V
DyLight 488 Labeled NB21-1052G
DyLight 550 Labeled NB21-1052R
DyLight 650 Labeled NB21-1052C
FITC Labeled NB21-1052F

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Bioinformatics Tool for Histone H3 Antibody (NB21-1052)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1052). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot