![Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H2AX-p-Ser-119-Antibody-RM224-Western-Blot-NBP3-18533-img0001.jpg "Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.")
| Reactivity | Hu, VbSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, mIF |
| Clone | RM224 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Histone H2AX [p Ser139] Antibody (RM224) (NBP3-18533) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal |
| Immunogen | A phospho-peptide corresponding to Histone H2AX [Ser139] |
| Modification | p Ser139 |
| Specificity | This antibody reacts to Histone H2AX only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H2AX |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H2AX Antibody (NBP3-18533)Find related products by research area.
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| Gene Symbol | H2AX |
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-18533_rabbit-histone-h2ax-p-ser-139-mab-rm224-immunocytochemistry-immunofluorescence-189202523444711.png "Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
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