![Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.](http://images.novusbio.com/fullsize/Histone-H2AX-Antibody-RM214-Western-Blot-NBP3-18534-img0001.jpg "Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.")
| Reactivity | VbSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, mIF |
| Clone | RM214 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Histone H2AX Antibody (RM214) (NBP3-18534) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H2AX |
| Specificity | This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H2AX |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H2AX Antibody (NBP3-18534)Find related products by research area.
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| Gene Symbol | H2AX |
![Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-18534] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-18534_rabbit-histone-h2ax-mab-rm214-immunocytochemistry-immunofluorescence-189202523403513.png "Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-18534] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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